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Synthesis of 32P-labeled intermediates on the purine biosynthetic pathway

✍ Scribed by Ryan A. Mehl; Tadhg P. Begley

Publisher
John Wiley and Sons
Year
2002
Tongue
French
Weight
79 KB
Volume
45
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

A fast and efficient route to ^32^P‐labeled intermediates on the purine biosynthetic pathway is described. Adenosine kinase catalyzes the phosphorylation of 5‐aminoimidazole‐4‐carboxamide ribonucleoside (AICARs) to give [^32^P]‐5‐aminoimidazole‐4‐carboxamide ribonucleotide (AICAR). This was then hydrolyzed to [^32^P]‐4‐carboxy‐5‐aminoimidazole ribonucleotide (CAIR). Treatment of CAIR with N5‐CAIR mutase gave [^32^P]‐5‐aminoimidazole ribonucleotide (AIR) in milligram quantities. Copyright © 2002 John Wiley & Sons, Ltd.

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