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Labelled polypeptides. v. synthesis of α-melanotropin labelled with tritium on the tyrosine residue, via the dibromo intermediate

✍ Scribed by K. Nikolics; I. Teplán; K. Medzihradszky

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
492 KB
Volume
12
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

A novel synthesis of α‐melanotropin labelled with tritium on the tyrosine residue ([Tyr‐3, 5‐^3^H^2^] ‐α‐melanotropin) is described. First, a precursor peptide, containing 3,5‐di‐bromotyrosine in position 2 ([Dbt^2^]‐α‐melanotropin) is prepared, which can be converted into the labelled hormone by catalytic tritiation in the last step of the synthesis. By this procedure the tedious work with highly active labelled intermediates, and the storage of the tritiated compound. usually accompanied by significant autoradiolysis, can be avoided.

📜 SIMILAR VOLUMES

Labelled polypeptides, III. Synthesis of
Labelled polypeptides, III. Synthesis of α-melanotropin labelled with tritium on the tyrosine residue
✍ K. Medzihradszky; K. Nikolics; I. Teplan; J. Seprodi; S. Fittkau 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 360 KB

## Abstract The synthesis and purification of α‐melanotropin labelled with tritium on the tyrosine residue is described. The hormone possesses a specific radioactivity of 2.8 Ci/mmole, which enables its application in studying transport processes, metabolism and mechanism of hormone action.