## Abstract A novel synthesis of α‐melanotropin labelled with tritium on the tyrosine residue ([Tyr‐3, 5‐^3^H^2^] ‐α‐melanotropin) is described. First, a precursor peptide, containing 3,5‐di‐bromotyrosine in position 2 ([Dbt^2^]‐α‐melanotropin) is prepared, which can be converted into the labelled
Labelled polypeptides, III. Synthesis of α-melanotropin labelled with tritium on the tyrosine residue
✍ Scribed by K. Medzihradszky; K. Nikolics; I. Teplan; J. Seprodi; S. Fittkau
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- French
- Weight
- 360 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
The synthesis and purification of α‐melanotropin labelled with tritium on the tyrosine residue is described. The hormone possesses a specific radioactivity of 2.8 Ci/mmole, which enables its application in studying transport processes, metabolism and mechanism of hormone action.
📜 SIMILAR VOLUMES
Catalytic reduction of 3j3-hydroxyandrost-4-ene-l7-one (I) with tritium gas gave a mixture of 3B-hydroxy-5a-androstane-17-one (11) and 3~-hydroxy-5cc-androstane-l7-one ( 1 1 1 ) . Oxidation of compound 1 1 followed by alkaline equilibration resulted in 37 % loss of label from position 4. 5a-Androsta