✦ LIBER ✦

Synthesis of 25-hydroxy[26,27-3H]vitamin D3 with high specific activity

✍ Scribed by Joseph L. Napoli; Mary A. Fivizzani; Alan J. Hamstra; Heinrich K. Schnoes; Hector F. DeLuca

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 556 KB
Volume: 96
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90610-9

No coin nor oath required. For personal study only.

✦ Synopsis

A synthesis of radiochemically pure 25-hydroxy[26,27-3H]vitamin D, with a specific activity of 160 Wmmol is reported. The structure and biological activity of the radiolabeled compound was verified by comigration on high-pressure liquid chromatography with synthetic 2Shydroxyvitamin D, to constant specific activity, and by conversion in vitro to la,25-dihydroxy[26,27-3H]vitamin D, with the chick kidney la-hydroxylase.

📜 SIMILAR VOLUMES

Synthesis of 25-hydroxy-[26,27-3H]vitami

Synthesis of 25-hydroxy-[26,27-3H]vitamin D2, 1,25-dihydroxy-[26,27-3H]vitamin D2 and their (24R)-epimers

✍ Rafal R. Sicinski; Yoko Tanaka; Mary Phelps; Heinrich K. Schnoes; Hector F. DeLu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 573 KB

Synthesis of a C-24-epimeric mixture of 25-hydroxy-[26,27-3H]vitamin D2 and a C-24-epimeric mixture of 1,25-dihydroxy-[26,27-'HIvitamin D2 by the Grignard reaction of the corresponding 25-keto-27-nor-vitamin D2 and lo-acetoxy-25keto-27-nor-vitamin D3 with tritiated methyl magnesium bromide is descri

Synthesis of 25-hydroxy[23,24-3H]vitamin

Synthesis of 25-hydroxy[23,24-3H]vitamin D3

✍ S. Yamada; H.K. Schnoes; H.F. DeLuca 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 534 KB

A synthesis of 25hydroxy [23,24JH]vitamin D3 leading to a radiochemically pure product with a specific activity of 78 Wmmol is described. The structure of the product was confirmed by comparison with unlabeled material and its biological activity was established by in vitro conversion to la,25dihydr

Synthesis of 2-deoxy-D-glucose [2,6,6′-3

Synthesis of 2-deoxy-D-glucose [2,6,6′-3H] with high specific activity

✍ J. Deschamps; B. Rousseau; J. P. Beaucourt 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 French ⚖ 257 KB

Suimnar : A new t r i t i a t i o n method was used t o obtain 2-deoxy-D-glucose nT8-3H1 s t a r t i n g from a mixture o f protected bro o deoxy glucose derivatives. Intermediates were characterized by IH-and 9 H-NHR.

Synthesis of 3H-D-ala2-leu-enkephalin-ch

Synthesis of 3H-D-ala2-leu-enkephalin-chloromethyl ketone of high specific activity

✍ G. Tóth; J. Hepp; F. Sirokmán; K. Medzihradszky 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 French ⚖ 283 KB

Boc-Tyr-D-Ala-Gly was prepared by catalytic tritiation of protected, iodinated tripeptide. The protected tritiated tripeptide and the Phe-Leu-CH2C1 dipeptide were condensed by the mixed anhydride method. The p r o - 3 " tecting group was removed by HC1-methanol. The /JH/-DALECK had a specific activi

A one step synthesis of ring labelled me

A one step synthesis of ring labelled melatonin-3H with high specific activity

✍ Yu-Ying Liu; Martha Minich 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 French ⚖ 231 KB

## Abstract A mixture of brominated melatonin derivatives has been synthesized for use as starting material for preparation of ring tritium labelled melatonin by catalytic hydrogenolysis. The high specific activity obtained makes this product useful in radioimmunoassay studies.

A potentially general synthesis of high

A potentially general synthesis of high specific activity specifically labelled tritiated peptides: Synthesis of [D-3-(2-naphthyl)-[2,3-3H]alanine6]LHRH

✍ Howard Parnes; Emma J. Shelton 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 French ⚖ 623 KB

High specific activity (43.7 Ci/mmol) D-3-(2-naphthyl)- [ 2,3, -3H]alanine (l) was obtained by reduction of methyl 2-N-acetylamino-3-(2-naphthyl)acrylate (5) with carrier free tritium in the presence of (Ph3P)3RhCl followed by enzymatic resolution and hydrolysis. Reductions of 5. and other substrat