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Synthesis, hybridization, and nuclease resistance properties of 2′-O-aminooxyethyl (2′-O-AOE) modified oligonucleotides

✍ Scribed by Andrew M. Kawasaki; Martin D. Casper; Thazha P. Prakash; Sheri Manalili; Henri Sasmor; Muthiah Manoharan; P.Dan Cook

Book ID
104260499
Publisher
Elsevier Science
Year
1999
Tongue
French
Weight
221 KB
Volume
40
Category
Article
ISSN
0040-4039

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✦ Synopsis

The novel RNA mimic 2'-O-AOE has been incorporated into antisense oligonucleotides. This 2'-O-modification significantly enhances hybridization against target RNA, and furthermore, exhibits specificity for RNA over DNA. The nuclease resistance (SVPD) of 2'-O-AOE modified phosphodiester oligonucleotides is significantly higher than the unmodified DNA and comparable to the 2'-O-MOE oligonucleotides.

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Synthesis of 2′-O-[2-[(N,N-dialkylamino)oxy]ethyl]-modified oligonucleotides: hybridization affinity, resistance to nuclease, and protein binding characteristics
✍ Thazha P Prakash; Andrew M Kawasaki; Elena A Lesnik; Namir Sioufi; Muthiah Manoh 📂 Article 📅 2003 🏛 Elsevier Science 🌐 French ⚖ 226 KB

Synthesis of a series of 2 0 -O-[2-[(N,N-dialkylamino)oxy]ethyl]-modified 5-methyluridine nucleoside phosphoramidites and solid supports are described. Using these monomers, modified oligonucleotides containing phosphodiester linkages were synthesized in high yields. These modified oligonucleotides