An improved protocol for the synthesis of oligonucleotides synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD containing a 3ЈϪ3Ј phosphodiester linkage, for use in an alternate strand triple-helix formation approach, is reported. spectroscopy. T
Synthesis and Triple Helix Formation by Alternate Strand Recognition of Oligonucleotides Containing 3‘-3‘ Phosphodiester Bonds
✍ Scribed by De Napoli, Lorenzo; Messere, Anna; Montesarchio, Daniela; Pepe, Antonietta; Piccialli, Gennaro; Varra, Michela
- Book ID
- 126227002
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 181 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0022-3263
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## Abstract Oligonucleotides with a 3′−3′ inversion of polarity, containing an acridine moiety attached to the nucleotide base flanking the 3′−3′ phosphodiester bond, have been synthesised, characterised and used to form alternate‐strand triple helix complexes. These have been investigated by UV me
Protected forms of 1,2,3-propanetriol and cis, cis-l,3,5-cyclohexanetriol were incorporated onto solid supports which were exploited in the solid phase synthesis of 3'-3' linked oligodeoxyribonucleotides (ODNs), involving only nucleoside 3'-phosphoramidites as building blocks. UV thermal denaturatio