A total of 13 metabolites thought to be possibly inhibitory were tested for their influence on PGM isozyme activities, each at several different concentrations. The analysis of statistical significance was based on enzyme activities obtained by densitometric measurements of starch gels. Five of the
Substrate affinity in PGM1, PGM2, and PGM3isozymes
✍ Scribed by G. Siebert; H. Ritter; J. Kömpf
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 197 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
An easy method for routine detection of PGM1, PGM2, and PGM3 isozymes is given. Differences in substrate affinity are discussed. Gene products pgm1 can be differentiated from gene products pgm3 by cofactor requirement.
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The existence of four alleles of phosphoglucomutase (PGM1) in human red cell lysates has previously been demonstrated by isoelectric focusing (Bark et al., 1976; Kühnl et al., 1977; Sutton and Burgess, 1978). Experiments are now described in which the position of each of the first-locus (PGM1) and s
The polymorphism of the human phosphoglucomutase isozyme PGM3 can be demonstrated in erythrocyte hemolysates by means of horizontal starch gel electrophoresis. Gene frequencies from southwestern Germany are given. The frequency of the allel PGM-23 was estimated to be 0.232.
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