The technique of isoelectric focusing on methylated 'cellogel' strips (CAGIF) was used to confirm the presence of four alleles of PGM1 in human red cell lysates. The subtypes of PGM1 were determined in two Polish population samples, from Southwestern Poland (WrocΕaw region, n=321) and Southeastern P
Characterisation of the isoenzymes of phosphoglucomutase (PGM) determined by the first (PGM1) and second (PGM2) locus observed by isoelectric focusing
β Scribed by J. G. Sutton
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 592 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
The existence of four alleles of phosphoglucomutase (PGM1) in human red cell lysates has previously been demonstrated by isoelectric focusing (Bark et al., 1976; KΓΌhnl et al., 1977; Sutton and Burgess, 1978). Experiments are now described in which the position of each of the first-locus (PGM1) and second-locus (PGM2) isoenzymes is defined, thus extending and confirming the original proposal made by Bark et al.
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A new variant of the PGMa1 erythrocyte enzyme system not identical with the known variants of the system has been detected in the hemolyzed red blood cells of a healthy blood donor by isoelectric focusing. Using this technique the variant is represented by two bands, a strong and slow one more catho
PGM1, ESD, and ACP were determined in bloodstain extracts by isoelectric focusing (IEF) with carrier ampholytes (CA) and HIEF. HIEF yields superior results in PGM typing from bloodstain extracts, whereas for ESD and ACP typing isoelectric focusing with carrier ampholytes seems to be the method of ch