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Studies on the spectrophotometric determination of DNA hybridization from renaturation rates

โœ Scribed by Huss, Volker A.R.; Festl, Herbert; Schleifer, Karl Heinz


Book ID
120734749
Publisher
Elsevier Science
Year
1983
Tongue
German
Weight
887 KB
Volume
4
Category
Article
ISSN
0723-2020

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## Abstract Fluorescence depolarization was used to measure the rate of renaturation of T2 DNA, which had been modified by chloroacetaldehyde. Rates were measured on DNA samples with 5โ€“15% of the base pairs modified and were found to agree with rates determined by DNA absorbance kinetics at 260 nm.

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## Abstract The effect of solvent viscosity on DNA renaturation rates has been investigated as a function of temperature for a number of solvent systems. The results are all consistent with a microscopic viscosity limitation of the rate determining step. Rates of renaturation in perchlorate and qua