๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Studies on the purification of vaccinia virus

โœ Scribed by M. D. Orlando; J. M. Riley; W. C. Patrick III

Publisher
John Wiley and Sons
Year
1964
Tongue
English
Weight
369 KB
Volume
6
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

Various methods for the removal of extraneous matter from vaccinia virus suspensions are described and compared. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high virus titers in embryonated eggs and high infectivity for mice. Although both procesaes resulted in products with the same titers, the differentially centrifuged product was approximately tenfold more purified as shown by the percentage solids of the suspensions before freeze drying.

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