✦ LIBER ✦
Rapid lysis of vaccinia virus on neutral sucrose gradients with release of intact DNA
✍ Scribed by J.Rodney Parkhurst; Charles Heidelberger
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 425 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A new and improved procedure has been developed for the isolation of intact DNA genomes from purified vaccinia virions. Purified virions are layered on a neutral sucrose gradient containing sodium dodecyl sulfate, 2-mercaptoethanol and sodium chloride at neutral pH. Intact viral DNA free from protein and fully sensitive to DNase I is rapidly released from the virions.