## Abstract MS2 RNA, which sediments at 27S in a neutral buffer, can be converted to a compact 57S conformation at pH 3.8. Requirements for this conversion, besides protonation, are small concentrations of Mg^++^ ions and a low ionic strength. On the other hand, after heating in the presence of EDT
Studies on the bacteriophage MS 2
β Scribed by Vandamme, E. ;Remaut, E. ;van Montagu, M. ;Fiers, W.
- Book ID
- 104695699
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 613 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0026-8925
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π SIMILAR VOLUMES
## Abstract When MS2 RNA is heated at low p^H^ in the presence of formaldehyde, a fastβsedimenting conformation is irreversibly formed. This species is homogeneous and stable at neutral p^H^. Its formation further requires Mg^++^ ions and low ionic strength. The most compact form sediments at 46S a
## Abstract The molecular weight of native 26.6S MS2 RNA, of acidic 53.1S MS2 RNA and of formaldehydeβtreated 36.1S MS2 RNA was determined by lightβscattering studies. The three forms examined correspond to monomers. Also Ξ½v values were determined, and in conjunction with our previous estimates for
## Abstract Reaction of 27 SMS2 RNA with formaldehyde normally results in an unfolded, 13.4 S form. At acidic pH, however, and under the proper ionic conditions, a compact component sedimenting at 36β40 S was obtained. The molecular weight of this species corresponds to a dinner. The formaldehyde c