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Studies on the bacteriophage MS2. XXII. Conformation of MS2 RNA in acid medium

✍ Scribed by H. Slegers; W. Fiers

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
754 KB
Volume
12
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

MS2 RNA, which sediments at 27S in a neutral buffer, can be converted to a compact 57S conformation at pH 3.8. Requirements for this conversion, besides protonation, are small concentrations of Mg^++^ ions and a low ionic strength. On the other hand, after heating in the presence of EDTA and at low ionic strength, the RNA can be unfolded to an 11.7S form at pH 6.8 and to 10.5S at pH 3.8. The compact 57S form has lost at least 50% of its secondary structure, as determined by its hypochromicity. It corresponds to a monomer species, as will be shown in a following paper (XXIV). Comparative studies with the homopolymers poly A and poly C and with the heteropolymers poly A,U, poly A,C, and poly A,G indicate that the interactions involved in the acid RNA conformation are not simply explainable by the known interactions of the A–A^+^, C–C^+^, and/or A–C^+^ type.

📜 SIMILAR VOLUMES

Studies on the bacteriophage MS2. XXIII.
Studies on the bacteriophage MS2. XXIII. Fixation of the MS2 RNA acid structure by formaldehyde
✍ H. Slegers; W. Fiers 📂 Article 📅 1973 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 468 KB

## Abstract When MS2 RNA is heated at low p^H^ in the presence of formaldehyde, a fast‐sedimenting conformation is irreversibly formed. This species is homogeneous and stable at neutral p^H^. Its formation further requires Mg^++^ ions and low ionic strength. The most compact form sediments at 46S a

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✍ H. Slegers; J. Clauwaert; W. Fiers 📂 Article 📅 1973 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 519 KB

## Abstract The molecular weight of native 26.6S MS2 RNA, of acidic 53.1S MS2 RNA and of formaldehyde‐treated 36.1S MS2 RNA was determined by light‐scattering studies. The three forms examined correspond to monomers. Also νv values were determined, and in conjunction with our previous estimates for

Studies on bacteriophage MS2. VIII. Evid
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✍ H. Slegers; W. Fiers 📂 Article 📅 1970 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 957 KB

## Abstract Reaction of 27 SMS2 RNA with formaldehyde normally results in an unfolded, 13.4 S form. At acidic pH, however, and under the proper ionic conditions, a compact component sedimenting at 36–40 S was obtained. The molecular weight of this species corresponds to a dinner. The formaldehyde c