𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Studies on the bacteriophage MS2. XXIV. Hydrodynamic properties of the native and acid MS2 RNA structures

✍ Scribed by H. Slegers; J. Clauwaert; W. Fiers

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
519 KB
Volume
12
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The molecular weight of native 26.6S MS2 RNA, of acidic 53.1S MS2 RNA and of formaldehyde‐treated 36.1S MS2 RNA was determined by light‐scattering studies. The three forms examined correspond to monomers. Also νv values were determined, and in conjunction with our previous estimates for S~20,W~ and [η], led again to the conclusion that formaldehyde‐cross‐linked 37S MS2 RNA is a monomer. On the basis of the experimentally observed R~G~, [η], S~20,W~, and νv values, and taking a prolate ellipsoid as a model, the hydrodynamic volume, the solvation water and the axial ratio could be deduced for the three conformations. In the “native” 26S form the RNA occupies only 8.2% of the hydrodynamic volume. The formation of the 36S and the 53S structures correspond largely to a loss of solvation water.

📜 SIMILAR VOLUMES

Studies on the bacteriophage MS2. XXII.
Studies on the bacteriophage MS2. XXII. Conformation of MS2 RNA in acid medium
✍ H. Slegers; W. Fiers 📂 Article 📅 1973 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 754 KB

## Abstract MS2 RNA, which sediments at 27S in a neutral buffer, can be converted to a compact 57S conformation at pH 3.8. Requirements for this conversion, besides protonation, are small concentrations of Mg^++^ ions and a low ionic strength. On the other hand, after heating in the presence of EDT

Studies on the bacteriophage MS2. XXIII.
Studies on the bacteriophage MS2. XXIII. Fixation of the MS2 RNA acid structure by formaldehyde
✍ H. Slegers; W. Fiers 📂 Article 📅 1973 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 468 KB

## Abstract When MS2 RNA is heated at low p^H^ in the presence of formaldehyde, a fast‐sedimenting conformation is irreversibly formed. This species is homogeneous and stable at neutral p^H^. Its formation further requires Mg^++^ ions and low ionic strength. The most compact form sediments at 46S a

Studies on bacteriophage MS2. VIII. Evid
Studies on bacteriophage MS2. VIII. Evidence for dimer formation of MS2 RNA by reaction with formaldehyde at acidic pH
✍ H. Slegers; W. Fiers 📂 Article 📅 1970 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 957 KB

## Abstract Reaction of 27 SMS2 RNA with formaldehyde normally results in an unfolded, 13.4 S form. At acidic pH, however, and under the proper ionic conditions, a compact component sedimenting at 36–40 S was obtained. The molecular weight of this species corresponds to a dinner. The formaldehyde c