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Studies on murine sarcoma virus: II. Detection of group-specific antigens by immunofluorescence

✍ Scribed by Jean-Claude Chuat; Françoise Lasquellec; Anne-Marie L'hirondel; Michel Boiron

Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
852 KB
Volume
7
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The group‐specific (GS) antigen of murine tumor viruses was demonstrated by immunofluorescence in mouse cells recently infected by mouse sarcoma virus, strain Moloney (MSV‐M), with the serum of rats carrying long‐transplanted MSV‐M tumors. GS antigen was detected 15 h post‐infection and was also present in various mouse and rat cell lines chronically infected with murine tumor viruses. The antigen was strictly localized in the cytoplasm of infected cells and was also found in mouse and rat cells chronically infected by members of the two major subgroups of murine tumor viruses. Further, the sera employed were shown to contain exclusively GS antibodies and the tumors used for immunization were found by several techniques to be free of virus envelope (V) antigens after a given number of passages in vivo. V antigens were visible only at the cell membrane and the time course of appearance of both GS and V antigens in recently infected cells was parallel. In contrast, GS antigen was not observed in two hamster tumor lines transformed by MSV‐M.

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