Studies on mouse sarcoma virus. IV. The isoelectric point of the group-specific antigen

✍ Scribed by J. C. Chuat; C. Bernard; I. Laprevotte; C. Seban; M. Boiron

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: French
• Weight: 606 KB
• Volume: 12
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910120322

✦ Synopsis

Abstract

At least three classes of species‐specific, group‐specific (gsl) antigen‐bearing molecules were separated by isoelectric focusing of tween‐ether disrupted Moloney strain mouse sarcoma virus (M‐MSV) derived from a permanent producer rat cell line (78 A1). The bulk of gs1 antigen, as measured by complement‐fixation, was associated with a ^3^H‐uridine‐labelled component characterized by an isoelectric point (ip H) of 5.9 and a sedimentation coefficient of 2.7 S~w, 20~ Statistical evaluation showed the ipH value to be remarkably stable. Neither ipH nor content of ^3^H‐uridine‐label was altered by ribonuclease (RNase), in contrast to an ipH 5.0 component found occasionally. The other gs1 antigen‐bearing molecules were only minor components and included an RNase‐resistant, ipH 5.5 component, frequently observed as a shoulder on the major peak, and an inconstant, poorly labelled component with a more variable ipH value of about 6.6. The significance of these findings is discussed in view of previous reports on the subject and of recent information regarding the structure of murine tumor viruses.