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Studies on gluconate metabolism in Aspergillus niger

โœ Scribed by Lakshminarayana, K. ;Modi, V. V. ;Shah, V. K.

Publisher
Springer-Verlag
Year
1969
Weight
640 KB
Volume
66
Category
Article
ISSN
0003-9276

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โœฆ Synopsis

A citric acid accumulating strain of Aspergillus niger adapted to grow on gluconic acid lactone as sole carbon source was studied with regards to the enzymatic changes occurring during its adaptive growth and compared with those of the parent strain cultivated on sucrose.

Glucose oxidase, glucose dehydrogenase, gluconate dehydrogenase and enzymes of Entner Doudoroffpathway could not be detected in the strain. Glueonokinase was detected in the strain and its inducible nature was established. An adaptive increase of gluconokinase, phosphogluconate dehydrogenase and ribose phosphate isomerase was observed. While the levels of the above enzymes were significantly higher, that of glucose 6-phosphate dehydrogenase were significantly low indicating the repression of the enzyme. Levels of hexokinase and fructose diphosphate a]dolase remained more or less the same as that of parent strain. It was inferred from the results that gluconate metabolism occurs via the pentose phosphate pathway following initial phosphorylation.

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Studies on gluconate metabolism in Asper
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โœ Lakshminarayana, K. ;Modi, V. V. ;Shah, V. K. ๐Ÿ“‚ Article ๐Ÿ“… 1969 ๐Ÿ› Springer-Verlag โš– 367 KB

A strain of Aspergillus niger adapted to grow on gluconate is cultivated on a medium containing gluconic acid lactone as the sole carbon source. Nutritional requirements of the adapted mold were studied. The organism shows optimum growth at 30 ~ C in a medium containing per litre 20 g of gluconic ac

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