A citric acid accumulating strain of Aspergillus niger adapted to grow on gluconic acid lactone as sole carbon source was studied with regards to the enzymatic changes occurring during its adaptive growth and compared with those of the parent strain cultivated on sucrose. Glucose oxidase, glucose d
Studies on gluconate metabolism in Aspergillus niger
โ Scribed by Lakshminarayana, K. ;Modi, V. V. ;Shah, V. K.
- Publisher
- Springer-Verlag
- Year
- 1969
- Weight
- 367 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0003-9276
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โฆ Synopsis
A strain of Aspergillus niger adapted to grow on gluconate is cultivated on a medium containing gluconic acid lactone as the sole carbon source. Nutritional requirements of the adapted mold were studied. The organism shows optimum growth at 30 ~ C in a medium containing per litre 20 g of gluconic acid lactone, 800 mg of NH~NOs, 100 mg of MgSOa, 7H20 and 270 mg of I(H2PO ~ having pH 2.0. ~ycelial growth reaches its maximum on the third day. Comparative growth responses of the adapted and parent strains on glueonate, glucose and sucrose were studied.
๐ SIMILAR VOLUMES
Phosphogluconate dehydrogenase was purified about 20 fold from an adapted strain of Aspergillus niger cultivated on gluconate as the sole carbon source. The enzyme showed pH optimum between 7.0 to 7.6. The enzyme was activated by glycyl glycine buffer and magnesium ions. Mn ++, Ba++, Ca++ cysteine
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The most important means of elucidating the pathway(s) of metabolism is, probably, the actual identification of all the enzymes involved in the postulated pathway. As far as the authors are aware, there has been no systematic attempt to elucidate the metabolic pathways in spores of fungi, although c