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Studies on polyol metabolism in Aspergillus niger

✍ Scribed by Desai, B. M. ;Modi, V. V. ;Shah, V. K.

Publisher: Springer-Verlag
Year: 1969
Weight: 288 KB
Volume: 67
Category: Article
ISSN: 0003-9276
DOI: 10.1007/bf00413675

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Studies on polyol metabolism in Aspergil

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Sorbitol dehydrogenase has been purified about 26 fold from a strain of Aspergillus niger, growing on sorbitol as the sole source of carbon. An absolute specificity of this enzyme for sorbitol, fructose, NAD and NADtt was observed. The Km for sorbitol and fructose were found to be 9.8 X 10 -5 M and

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A strain of Aspergillus niger adapted to grow on gluconate is cultivated on a medium containing gluconic acid lactone as the sole carbon source. Nutritional requirements of the adapted mold were studied. The organism shows optimum growth at 30 ~ C in a medium containing per litre 20 g of gluconic ac

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A citric acid accumulating strain of Aspergillus niger adapted to grow on gluconic acid lactone as sole carbon source was studied with regards to the enzymatic changes occurring during its adaptive growth and compared with those of the parent strain cultivated on sucrose. Glucose oxidase, glucose d

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Phosphogluconate dehydrogenase was purified about 20 fold from an adapted strain of Aspergillus niger cultivated on gluconate as the sole carbon source. The enzyme showed pH optimum between 7.0 to 7.6. The enzyme was activated by glycyl glycine buffer and magnesium ions. Mn ++, Ba++, Ca++ cysteine

Polyol synthesis in Aspergillus niger: I

Polyol synthesis in Aspergillus niger: Influence of oxygen availability, carbon and nitrogen sources on the metabolism

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Polyol production has been studied in Aspergillus niger under different conditions. Fermentations have been run using high concentration of glucose or xylose as carbon source and ammonium or nitrate as nitrogen source. The growth of biomass, as freely dispersed hyphae, led to an increase of medium v