Studies of orotidine 5′-phosphate decarboxylase in Neurospora crassa
✍ Scribed by Thomas I. Pynadath; Robert M. Fink
- Book ID
- 118845660
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 464 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0003-9861
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A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'
We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecas