Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa
✍ Scribed by D.J. Ballance; F.P. Buxton; G. Turner
- Book ID
- 115757249
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 719 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'
The sequencing and comparison of the genes encoding the glyoxylate bypass enzyme malate synthase of Aspergillus nidulans (acuE) and Neurospora crassa (acu-9) are presented. The predicted amino acid sequences of the A. nidulans and N. crassa enzymes are 538 and 542 residues respectively and the prote