Cloning of orotidine-5′-phosphate decarboxylase (URA3) gene from sourdough yeastCandida milleriCBS 8195
✍ Scribed by Ha-Yeon Lee, Eun-Hee Park, Myoung-Dong Kim
- Book ID
- 118791418
- Publisher
- The Korean Society of Food Science and Technology
- Year
- 2012
- Tongue
- English
- Weight
- 538 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1226-7708
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The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to
The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the fam