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Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) ofCandida utilis. Comparison with the OMP decarboxylase gene family

✍ Scribed by Rodríguez, Luis; Chávez, Francisco P.; González, Maria E.; Basabe, Liliana; Rivero, Tanilo

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 265 KB
Volume: 14
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(199811)14:15<1399::aid-yea324>3.0.co;2-d

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✦ Synopsis

The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae.