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Increased activities of hepatic orotidine 5′-phosphate pyrophosphorylase and orotidine 5′-phosphate decarboxylase induced by orotate

✍ Scribed by Jürgen Pausch; Dietrich Keppler; Karl Decker

Book ID
115902578
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
216 KB
Volume
20
Category
Article
ISSN
0014-5793

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A positive selection for mutants lacking
A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
✍ Boeke, Jef D. ;La Croute, Francois ;Fink, Gerald R. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 236 KB

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow no