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A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

✍ Scribed by Boeke, Jef D. ;La Croute, Francois ;Fink, Gerald R.

Publisher: Springer
Year: 1984
Tongue: English
Weight: 236 KB
Volume: 197
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330984

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✦ Synopsis

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

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