A mutant yeast constitutive for synthesis of both dihydroorotic acid dehydrogenase and orotidine 5' phosphate decarboxylase has been isolated. This phenotype is due to a single gene unlinked to any of the five genes of the pyrimidine pathway. This gene, called ppr1, induces the unlinked genes ural a
✦ LIBER ✦
Dihydroorotate dehydrogenase, orotate phosphoribosyltransferase and orotidine-5′-phosphate decarboxylase in Plasmodium falciparum
✍ Scribed by A.M. Gero; K. Tetley; G.H. Coombs; R.S. Phillips
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 218 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0035-9203
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A rapid and efficient method is described for the synthesis of [6-r4C]orotidine 5'-monophosphate from radioactive erotic acid using purified yeast orotate phosphoribosyltransferase and inorganic pyrophosphatase. Radioactive orotidine 5'-monophosphate is purified by ion exchange chromatography and em