𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enzymatic synthesis of [6-14C]orotidine 5′-monophosphate and its use in the assay of orotate phosphoribosyltransferase and orotidylate decarboxylase

✍ Scribed by John M. Rawls Jr.

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
686 KB
Volume
86
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid and efficient method is described for the synthesis of [6-r4C]orotidine 5'-monophosphate from radioactive erotic acid using purified yeast orotate phosphoribosyltransferase and inorganic pyrophosphatase. Radioactive orotidine 5'-monophosphate is purified by ion exchange chromatography and employed in small scale assays ofDrosophila orotate phosphoribosyltransferase and orotidylate decarboxylase in which both enzyme activities are simultaneously measured in single reaction mixtures. Radioactive substrate and products are separated for counting using DEAE-cellulose paper chromatograms developed in one or two solvents.

📜 SIMILAR VOLUMES

One step conversion of glutarimide-2,6-1
One step conversion of glutarimide-2,6-14C to pentachloropyridine-2,6-14C and its subsequent use in the synthesis of 2-octyl (4-amino-3,5-dichloro-6-fluoro-2-pyridinyloxy-2,6-14C)acetate
✍ L. H. McKendry 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 French ⚖ 208 KB 👁 1 views

## Abstract A convenient method of preparing 2‐octyl (4‐amino‐3,5‐dichloro‐6‐fluoro‐2‐pyridinyloxy‐2,6‐^14^C)acetate from glutarimide‐2,6‐^14^C is described. The process includes a novel one‐step conversion of glutarimide‐2,6‐^14^C to pentachloropyridine‐2,6‐^14^C.

Directed lithiation of 3,5-dichloroanili
Directed lithiation of 3,5-dichloroaniline and its application in the synthesis of 1,3-dichloro-6,7,8,9,10,12-hexahydroazepino[2,1-b][5-14C]quinazoline monohydrochloride
✍ I. Victor Ekhato; Che C. Huang 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 421 KB

## Abstract 1,3‐Dichloro‐6,7,8,9,10,12‐hexahydroazepino[2,1‐b][5‐^14^C]quinazoline monohydrochloride (1) was synthesized in 12% overall yield starting from 3,5‐dichloroaniline, and a radiochemical purity of 99.6% was obtained. The synthetic sequence was facilitated by the regio‐controlled preparati

Synthesis of 14C-labelled hexahydro-1,3,
Synthesis of 14C-labelled hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), 2,4,6-trinitrotoluene (TNT), nitrocellulose (NC) and glycidylazide polymer (GAP) for use in assessing the biodegradation potential of these energetic compounds
✍ Guy Ampleman; Sonia Thiboutot; J. Lavigne; A. Marois; J. Hawari; A. M. Jones; D. 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 French ⚖ 756 KB

## Abstract Within the framework of an R & D project on bioremediation of soils contaminated with energetic compounds, the biodegradation of energetic products such as hexogen (RDX), trinitrotoluene (TNT), nitrocellulose (NC) and glycidyl azide polymer (GAP) is under study. Microcosm assays must be