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Cloning of thePYR4gene encoding orotidine-5′-phosphate decarboxylase inCephalosporium acremonium

✍ Scribed by Alejandro Vian; Miguel Angel Peñalva

Book ID: 104717250
Publisher: Springer-Verlag
Year: 1990
Tongue: English
Weight: 638 KB
Volume: 17
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00312613

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✦ Synopsis

We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.

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