Studies of in vivo mutations in rpsL transgene in UVB-irradiated epidermis of XPA-deficient mice
โ Scribed by Hiroaki Murai; Seiji Takeuchi; Yoshimichi Nakatsu; Minoru Ichikawa; Masafumi Yoshino; Yoichi Gondo; Motoya Katsuki; Kiyoji Tanaka
- Book ID
- 114070193
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 829 KB
- Volume
- 450
- Category
- Article
- ISSN
- 0027-5107
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A C-terminal 183 amino acid-truncated mutation of the mouse Xpg gene (XpgDeltaex15) gives rise to a partial deficiency in nucleotide excision repair in homozygously affected cells. We studied the effect of this mutation on UVB-induced mutagenesis in mouse skin, using transgenic mice harboring lambda
Ultraviolet light B (UVB)-induced mutagenesis was studied in gpt delta transgenic mice, which contain the EG10 shuttle vector as a transgene. The mice were exposed to UVB at single doses of 0.3, 0.5, 1.0, 1.5, and 2.0 kJ/m 2 . At 4 weeks after irradiation, the mutant frequencies (MF) of the gpt gene