## Abstract The isolated manno‐protein contains about 80% mannose and 10% glucose. Methylation analysis established the highly branched nature of this polysaccharide and the presence of 1,2‐, 1,3‐ and 1,6‐linkages, as well as the linkages of the branchpoints. The research of the acetolysis fragment
Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. VI. Isolierung und Strukturaufklärung der Glucane
✍ Scribed by H. D. Grimmecke; Prof. Dr. G. Reuter
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 502 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
Alkali‐soluble glucan was obtained from Candida spec. H by extraction with dilute sodium hydroxide. This minor component is quite heterogeneous in molecular size, has a highly branched structure and contains 19% β(1,3)‐, 24% β(1,6)‐ and only 2% β(1,2)‐linkages
Other minor polysaccharide components have been isolated by acetic acid extraction. They have been identified as glycogen and linear β(1,6)‐glucan, respectively
The major component is a 1,2‐, 1,3‐, 1,6‐glucan branched in 1,2,3‐, 1,3,6‐ and 1,2,6‐positions, containing chitin. So we can confirm that different yeasts may differ in glucan structures which vary considerably both in the degree of branching and molecular size.
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## Abstract Mild alkaline degradation of the proteophosphomannan (PPM) under conditions that effect β‐elimination of the residues, O‐glycosidically bound on serine and threonine, release mainly mannose, glucose, mannobioses and mannotrioses with the same structures as those obtained by acetolysis o
## Abstract The ^31^P‐NMR spectra of the proteophosphomannan (PPM) and also that of mildly hydrolyzed PPM demonstrated phosphomonoester (in both preparations), acid labile and acid stable phosphodiester linkage, and polyphosphate. Decreasing in size by pronase digestion, separation, purification an
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