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Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. IV. Struktur der alkalilabilen Oligosaccharide im Mannan-Protein-Phosphat-Komplex

✍ Scribed by H.-D Grimmecke; Prof. Dr. G. Reuter

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
443 KB
Volume
21
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

Mild alkaline degradation of the proteophosphomannan (PPM) under conditions that effect β‐elimination of the residues, O‐glycosidically bound on serine and threonine, release mainly mannose, glucose, mannobioses and mannotrioses with the same structures as those obtained by acetolysis of the polysaccharide component of the PPM. Methylation analysis and paper chromatography demonstrated the structural heterogeneity of the tetra‐, penta‐ and hexasaccharide fractions containing 1,2‐ and 1,6‐linked and 1,2,6‐branched manno‐oligosaccharides. Methylation analysis and acetolysis, paper chromatography and analysis of the carbohydrate composition of the oligosaccharide fractions DP 7–12 and DP 15–19 demonstrated inner core region like structures containing 1,2‐ and 1,6‐linked mannose, 1,2,6‐ and 1,3,6‐branchpoints and N‐acetyl‐d‐glucosamine.

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## Abstract The ^31^P‐NMR spectra of the proteophosphomannan (PPM) and also that of mildly hydrolyzed PPM demonstrated phosphomonoester (in both preparations), acid labile and acid stable phosphodiester linkage, and polyphosphate. Decreasing in size by pronase digestion, separation, purification an

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## Abstract The isolated manno‐protein contains about 80% mannose and 10% glucose. Methylation analysis established the highly branched nature of this polysaccharide and the presence of 1,2‐, 1,3‐ and 1,6‐linkages, as well as the linkages of the branchpoints. The research of the acetolysis fragment

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## Abstract The proteophosphomannan (PPM) obtained by extraction with citrate buffer, purification by the cetavlon method and gelfiltration contains a high proportion of diesterfied phosphate groups between the C‐6 of a side chain mannose and another glycosidically bound carbohydrate. Mild acid hyd

Struktur der Zellwandpolysaccharide in d
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## Abstract Alkali‐soluble glucan was obtained from __Candida spec.__ H by extraction with dilute sodium hydroxide. This minor component is quite heterogeneous in molecular size, has a highly branched structure and contains 19% β(1,3)‐, 24% β(1,6)‐ and only 2% β(1,2)‐linkages Other minor polysacch