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Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. II. Charakterisierung der Bindung des Phosphats am Mannan-Protein-Phosphat-Komplex und Identifizierung der als Phosphodiester gebundenen Mono- und Oligosaccharide

✍ Scribed by H. D. Grimmecke; Prof. Dr. G. Reuter

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
462 KB
Volume
21
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The proteophosphomannan (PPM) obtained by extraction with citrate buffer, purification by the cetavlon method and gelfiltration contains a high proportion of diesterfied phosphate groups between the C‐6 of a side chain mannose and another glycosidically bound carbohydrate. Mild acid hydrolysis cleaved the phosphate diester linkages to yield mono‐ and oligosaccharide fractions. Chemical analysis and ^1^H‐NMR studies demonstrated the heterogeneity of the oligosaccharide fractions containing α(1,2)‐, α(1,3)‐ and α(1,6)‐linked manno‐oligosaccharides. All repeated PPM preparations contained mono‐, tri‐ and heptasaccharides as the phosphate bound main compounds. Galactose, arabinose, fucose, and rhamnose of the monosaccharide fraction and tetra‐, penta‐ and hexasaccharides were not found in all preparations.

📜 SIMILAR VOLUMES

Struktur der Zellwandpolysaccharide in d
Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. IV. Struktur der alkalilabilen Oligosaccharide im Mannan-Protein-Phosphat-Komplex
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## Abstract Mild alkaline degradation of the proteophosphomannan (PPM) under conditions that effect β‐elimination of the residues, O‐glycosidically bound on serine and threonine, release mainly mannose, glucose, mannobioses and mannotrioses with the same structures as those obtained by acetolysis o

Struktur der Zellwandpolysaccharide in d
Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. III. Charakterisierung unterschiedlicher Phosphatbindungen im Mannan-Protein-Phosphat-Komplex
✍ H. D. Grimmecke; H. Meyer; D. Scheller; Prof. Dr. G. Reuter 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 594 KB

## Abstract The ^31^P‐NMR spectra of the proteophosphomannan (PPM) and also that of mildly hydrolyzed PPM demonstrated phosphomonoester (in both preparations), acid labile and acid stable phosphodiester linkage, and polyphosphate. Decreasing in size by pronase digestion, separation, purification an