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Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. III. Charakterisierung unterschiedlicher Phosphatbindungen im Mannan-Protein-Phosphat-Komplex

✍ Scribed by H. D. Grimmecke; H. Meyer; D. Scheller; Prof. Dr. G. Reuter

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
594 KB
Volume
21
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The ^31^P‐NMR spectra of the proteophosphomannan (PPM) and also that of mildly hydrolyzed PPM demonstrated phosphomonoester (in both preparations), acid labile and acid stable phosphodiester linkage, and polyphosphate. Decreasing in size by pronase digestion, separation, purification and characterization of the high and low molecular phosphates by ^31^P‐NMR spectroscopy and chemical analysis revealed the mannan protein is phosphorylated in the N‐glycosidically linked carbohydrate parts and in the O‐glycosidically linked oligosaccharides. Another phosphate serves as a bridge between the serine of the protein and mannose, mannobioses and mannotrioses and between the threonine and a lipophilic acylglycerid unit. The origin of the polyphosphates has been discussed.

📜 SIMILAR VOLUMES

Struktur der Zellwandpolysaccharide in d
Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. IV. Struktur der alkalilabilen Oligosaccharide im Mannan-Protein-Phosphat-Komplex
✍ H.-D Grimmecke; Prof. Dr. G. Reuter 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 443 KB

## Abstract Mild alkaline degradation of the proteophosphomannan (PPM) under conditions that effect β‐elimination of the residues, O‐glycosidically bound on serine and threonine, release mainly mannose, glucose, mannobioses and mannotrioses with the same structures as those obtained by acetolysis o

Struktur der Zellwandpolysaccharide in d
Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. I. Struktur des alkalistabilen Mannan-Proteins
✍ H. D. Grimmecke; Prof. Dr. G. Keuter 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 663 KB

## Abstract The isolated manno‐protein contains about 80% mannose and 10% glucose. Methylation analysis established the highly branched nature of this polysaccharide and the presence of 1,2‐, 1,3‐ and 1,6‐linkages, as well as the linkages of the branchpoints. The research of the acetolysis fragment

Struktur der Zellwandpolysaccharide in d
Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. II. Charakterisierung der Bindung des Phosphats am Mannan-Protein-Phosphat-Komplex und Identifizierung der als Phosphodiester gebundenen Mono- und Oligosaccharide
✍ H. D. Grimmecke; Prof. Dr. G. Reuter 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 462 KB

## Abstract The proteophosphomannan (PPM) obtained by extraction with citrate buffer, purification by the cetavlon method and gelfiltration contains a high proportion of diesterfied phosphate groups between the C‐6 of a side chain mannose and another glycosidically bound carbohydrate. Mild acid hyd