The exopolysaccharide produced by Lactobacillus helveticus 766 in skimmed milk was found to be composed of D-glucose and D-galactose in a molar ratio of 2:1. Linkage analysis and 1D/2D NMR studies (tH and 13C) performed on the native polysaccharide, and on oligosaccharides obtained from a partial ac
Structure of the major exopolysaccharide produced by Azotobacter beijerinckii B-1615
β Scribed by Leonid M. Likhosherstov; Sofia N. Senchenkova; Alexander S. Shashkov; Varvara A. Derevitskaya; Irina V. Danilova; Irina V. Botvinko
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 388 KB
- Volume
- 222
- Category
- Article
- ISSN
- 0008-6215
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β¦ Synopsis
A. beijerinckii strain B-1615 produced two acidic exopolysaccharides in the ratio approximately 9:1. The minor polysaccharide contained mannuronic and guluronic acids in the ratio 2.3:1 and is a bacterial alginate. The major polysaccharide consisted of D-galactose, L-rhamnose, and pyruvic acid in the ratios 2:1:1 and was acetylated. On the basis of methylation analysis, and 1H- and 13C-n.m.r. spectroscopy of the polysaccharide before and after removal of the pyruvic acid residues and O-deacetylation, it was concluded that the major polysaccharide had the structure [formula; see text] with up to 1.5 OAc groups per repeating unit.
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## Abstract A method is described for constructing a conformational model in water of a heteropolysaccharide built up from repeating units, and is applied to the exopolysaccharide produced by __Lactobacillus helveticus__ 766. The molecular modeling method is based on energy minima, obtained from mo
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