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The structure of the acidic exopolysaccharide produced by Pseudomonas “gingeri” strain Pf9

✍ Scribed by Paola Cescutti; Stanley F. Osman; William F. Fett; David Weisleder

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
413 KB
Volume
275
Category
Article
ISSN
0008-6215

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✦ Synopsis

The structure of the acidic exopolysaccharide produced by the mushroom pathogen Pseudomonas "gingeri" strain Pf9, a bacterium which causes ginger blotch, was investigated by chemical analysis, mass spectrometry and 1D and 2D NMR spectroscopy. The polysaccharide consists of the linear trisaccharide repeating unit [formula: see text] where the cyclic pyruvic acetal groups at O-4 and O-6 of the mannopyranosyl residues have the S-configuration. Methylation analysis under neutral conditions and NMR data showed that the mannose residues are acetylated at O-2. This exopolysaccharide has the same structure as the E. coli K55 capsular polysaccharide and differs from the Klebsiella K5 capsular polysaccharide only in the position of acetylation (C-2 of the glucopyranose residue).

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