✦ LIBER ✦

Structure of the acidic extracellular gelling polysaccharide produced by Pseudomonas elodea

✍ Scribed by Malcolm A. O'Neill; Robert R. Selvendran; Victor J. Morris

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 665 KB
Volume: 124
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(83)88360-8

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✦ Synopsis

The gelling polysaccharide secreted by the bacterium Pseudomonas elodea contains L-rhamnose, D-glucose, and D-glt.tCUrOniC acid in the molar ratios 1:2:1. Methylation analysis of native and carboxyl-reduced polysaccharide indicated (l-+4)-Rhap, (1+3)-Glcp, (1+4)-Glcp, and (l-4)-GlcpA to be present in the ratios 1: 1: 1: 1. Graded acid hydrolysis gave a series of acidic oligosaccharides that were isolated by ion-exchange chromatography and fractionated by gel-filtration. The purified oligosaccharides were analysed and characterised as their methylated alditol derivatives by e.i.-m.s. and c.i.-m.s., and also examined by fast-atom-bombardment (f.a.b.)-m.s. The tetrasaccharide repeating-unit 1, excluding acetyl groups, is proposed.

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