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Structure and expression of a tobacco β-1,3-glucanase gene

✍ Scribed by Masuru Ohme-Takagi; Hideaki Shinshi

Publisher
Springer
Year
1990
Tongue
English
Weight
770 KB
Volume
15
Category
Article
ISSN
0167-4412

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✦ Synopsis

We determined the primary structure of a tobacco r-1,3-glucanase gene. The r-1,3-glucanase gene has a single large intron, and the intron separates coding regions of the signal peptide and the mature enzyme. Analysis of the 5'-flanking region sequence revealed an 11 bp GC-rich element with perfect homology to the putative regulatory sequence of tobacco chitinase genes. RNA blot analysis showed that levels of mRNAs of r-1,3-glucanase and chitinase are coordinately increased in response to ethylene and salicylic acid. Accumulation of fl-l,3-glucanase mRNA in suspension-cultured cells is rapidly induced at late logarithmic growth phase. Members of the tobacco r-1,3-glucanase gene families are classified into two subfamilies. One of the subfamilies appeared to be transcriptionally inactive.

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