Developmental and hormonal regulation of β-1,3-glucanase in tobacco

✍ Scribed by G. Felix; F. Meins

• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 621 KB
• Volume: 167
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00391416

✦ Synopsis

A highly sensitive and specific "rocket" immunoassay was used to measure the content of an endo-type/?-l,3-glucanase (EC 3.2.1.39) in tissues of Nicotiana tabacum L. cv. Havana 425. We show that the accumulation of fl-l,3-glucanase in cultured pith-parenchyma tissue is blocked by combinations of the auxin, c~-naphthaleneacetic acid (NAA), and the cytokinin, kinetin. When tissues pre-incubated for 7 d on complete medium containing 2.0 rag. 1-1 NAA and 0.3 rag. 1-1 kinetin are transferred onto medium without hormones or with either hormone added separately, the/?-1,3glucanase content expressed per mg soluble protein increases approx, ten fold over a 7-d period. Under these inductive conditions, up to approx. 5% of the soluble protein is/?-l,3-glucanase. The induction is inhibited by >90% when tissues are cultured over the same period on medium containing both hormones. This /?-l,3-glucanase is developmentally regulated in the intact plant. It is a major component of the soluble protein in the lower leaves and roots but is not detectable in leaves near the top of the plant.