𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Characterization and expression of chitinase and 1,3-β-glucanase genes in cotton

✍ Scribed by Richard L. Hudspeth; Susan L. Hobbs; David M. Anderson; John W. Grula

Publisher
Springer
Year
1996
Tongue
English
Weight
559 KB
Volume
31
Category
Article
ISSN
0167-4412

No coin nor oath required. For personal study only.

✦ Synopsis

We have isolated cDNA clones representing mRNAs encoding chitinase and 1,3-fl-glucanase in cotton (Gossypium hirsutum L.) leaves. The chitinase clones were sequenced and found to encode a 28,806 Da protein with 71% amino acid sequence similarity to the SK2 chitinase from potato (Solanum tuberosum). The 1,3-fl-glucanase clones encoded a 37,645 Da protein with 57.6% identity to a 1,3-fl-glucanase from soybean (Glycine max). Northern blot

📜 SIMILAR VOLUMES

Structure and expression of a tobacco β-
Structure and expression of a tobacco β-1,3-glucanase gene
✍ Masuru Ohme-Takagi; Hideaki Shinshi 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 770 KB

We determined the primary structure of a tobacco r-1,3-glucanase gene. The r-1,3-glucanase gene has a single large intron, and the intron separates coding regions of the signal peptide and the mature enzyme. Analysis of the 5'-flanking region sequence revealed an 11 bp GC-rich element with perfect h

Cloning and characterization of 1,3-β-gl
Cloning and characterization of 1,3-β-glucanase-encoding genes from non-conventional yeasts
✍ Esteban, Pedro F.; Vazquez de Aldana, Carlos R.; del Rey, Francicso 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 530 KB

The molecular cloning of 1,3--glucanase-encoding genes from different yeast species was achieved by screening genomic libraries with DNA probes obtained by PCR-amplification using oligonucleotides designed according to conserved regions in the EXG1, EXG2 and SSG1 genes from Saccharomyces cerevisiae.