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Structural analysis of the heptose/hexose region of the lipopolysaccharide from escherichia coli K-12 strain W3100

✍ Scribed by Otto Holst; Ulrich Zähringer; Helmut Brade; Aleksander Zamojski

Book ID
102995991
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
810 KB
Volume
215
Category
Article
ISSN
0008-6215

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✦ Synopsis

The disaccharide L-glycero-D-manno-heptosyl-D-glucose was isolated from the lipopolysaccharide (LPS) of Escherichia coli K-12 strain W3100 after partial hydrolysis with acid, and the structure was determined by methylation analysis, n.m.r. spectroscopy, and comparison with a synthetic standard. In addition, the oligosaccharides L,D-Hep-D-Glc-D-Glc and L,D-Hep-D-Glc-D-Glc-D-Glc were isolated, and their structures were established by g.l.c.-m.s. and methylation analysis. The results indicated that L-glycero-D-manno-heptose, a characteristic constituent of the inner core region, may also occur in the outer core region which, in E. coli, is generally composed of hexoses. A revised structure of the carbohydrate backbone of the hexose/heptose region of the LPS is given.

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