A series of deletions, Mu insertions and point mutations affecting the malK-lamB operon have been isolated. They were used to establish a deletion map of this operon, which could be divided in 27 intervals, with 16 in malK and 11 in lamB. One interesting feature of this map is the lack of randomness
Structure of the malB region in Escherichia coli K12
β Scribed by Silhavy, Thomas J. ;Brickman, Edith ;Bassford, Philip J. ;Casadaban, Malcolm J. ;Shuman, Howard A. ;Schwartz, Vicki ;Guarente, Leonard ;Schwartz, Maxime ;Beckwith, Jonathan R.
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 1014 KB
- Volume
- 174
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Starting with a strain containing a malK-lacZ fusion, a series of lambda plaque-forming phages which carry varying amounts of the malE,F operon have been isolated. We have used these phages to construct a deletion map of the malE,F operon. The construction of this deletion map has led to the identification of a new gene, malG. The malG gene is located distal to malF. The malG gene product is a protein required for the active transport of maltose and maltodextrins.
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A correlation between the genetic and physical maps of the malB region was obtained by performing a restriction cleavage analysis of DNA's carrying various genetically characterized malB deletions. This also allowed to localize the boundaries between malF and malE, malE and malK, mal K and lamB on t
lamB, the structural gene for lambda receptor, is the second gene of the malK-lamB operon in the malB region of the Escherichia coli K12 chromosome. lamB is essentially not expressed in the absence of an active malT gene product, the activator of the maltose regulon. A malT strain is resistant to ph
A fine structure analysis of the threonine operon in Escherichia coli K-12 was performed by deletion mapping. Lambda transducing bacteriophages carrying various parts of the threonine operon were isolated from strains in which the lacZ gene was fused to a thr gene. We tested for recombination betwee
The regulatory region of the gua operon of Escherichia coli is contained within a 2.1 kb EcoR1 restriction fragment isolated from a lambda pgua transducing phage. This DNA fragment was inserted into pPV33-II, a promoter-cloning vector, where it activated the gene(s) for tetracycline resistance. The