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Fine structure analysis of the threonine operon in Escherichia coli K-12

✍ Scribed by Saint-Girons, Isabelle ;Margarita, Danielle

Publisher: Springer
Year: 1978
Tongue: English
Weight: 501 KB
Volume: 162
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00333856

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✦ Synopsis

A fine structure analysis of the threonine operon in Escherichia coli K-12 was performed by deletion mapping. Lambda transducing bacteriophages carrying various parts of the threonine operon were isolated from strains in which the lacZ gene was fused to a thr gene. We tested for recombination between deletions of the threonine promotor extending into the threonine operon, carried by the phage, and bacterial thr auxotrophs. The relative order of thrO (operator) mutations was established. We propose that an operator region is located between a promoter region and the structural genes. Mutations leading to the desensitization of the aspartokinase I-homoserine dehydrogenase I towards threonine were localized in two different regions of the thrA gene.

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