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New regulatory mutations affecting the expression of the threonine operon in Escherichia coli K-12

✍ Scribed by Saint-Girons, Isabelle

Publisher: Springer
Year: 1978
Tongue: English
Weight: 517 KB
Volume: 162
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00333855

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✦ Synopsis

The promoter of the threonine operon was joined to the structural genes of the lac operon in Escherichia coli K 12. The synthesis of beta-galactosidase was thus repressed by threonine plus isoleucine in the fusion strains. To isolate mutations which affect the expression of the threonine operon, alterations in the level of expression of the lacZ gene were selected. A new type of regulatory mutation was discovered.

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