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Increased efficiency of the outer membrane PhoE protein pore in escherichia coli K-12 mutants with heptose-deficient lipopolysaccharide

✍ Scribed by Jaap Korteland; Ben Lugtenberg


Book ID
115726682
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
587 KB
Volume
774
Category
Article
ISSN
0005-2736

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Gene encoding a hybrid OmpF-PhoE pore pr
✍ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat