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Role of the cell surface-exposed regions of outer membrane protein PhoE of Escherichia coli K12 in the biogenesis of the protein

✍ Scribed by Marja AGTERBERG; Henriëtte ADRIAANSE; Edwin TIJHAAR; Annelies RESINK; Jan TOMMASSEN


Book ID
115126536
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
579 KB
Volume
185
Category
Article
ISSN
1432-1327

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Gene encoding a hybrid OmpF-PhoE pore pr
✍ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat