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Insertion mutagenesis on a cell-surface-exposed region of outer membrane protein PhoE of Escherichia coli K-12

✍ Scribed by Marja AGTERBERG; Roland BENZ; Jan TOMMASSEN


Book ID
115125407
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
794 KB
Volume
169
Category
Article
ISSN
1432-1327

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To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat