Staining for protease activity on polyacrylamide gels
โ Scribed by K.R. Lynn; N.A. Clevette-Radford
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 139 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates
An activity stain for the detection of L-serine dehydratase in gels is described. After electrophoresis, the gel is incubated with L-serine, and the pyruvate formed is detected by its reaction with 0.6% 1,2-diamino4nitrobenzene in 2 M WC1 at 90ยฐC. A red band on a yellow background indicates the posi