Staining Technique for Phosphatases in Polyacrylamide Gels
โ Scribed by C. Queirozclaret; J.C. Meunier
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 398 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates
A new staining procedure for proteins in polyacrylamide gels has been developed. This procedure, utilizing Coomassie brilliant blue G250, is rapid (as quick as 30 min) and simple to perform, requires little or no destaining, and has a sensitivity of a least 1 pg of protein per band. It can be used t
Methods for the detection of RNase after polyacrylamide gel electrophoresis have required long incubation times or indirect techniques that allowed the enzyme ample time to diffuse into a broad band (l-3). Wolf (4) recently described a new technique in which the gel was incubated in a solution of lo