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Activity Staining of Protein Inhibitors of Proteases on Gelatin-Containing Polyacrylamide Gel Electrophoresis

✍ Scribed by R. Felicioli; B. Garzelli; L. Vaccari; D. Melfi; E. Balestreri

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
158 KB
Volume
244
Category
Article
ISSN
0003-2697

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A fast and convenient method for silver staining of proteins on electroblotting membranes was developed based on Gallyas' histochemical intensifier and applied to human endothelial cell proteins separated by one-and two-dimensional electrophoresis and electroblotted to polyvinyl difluoride membranes