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An activity stain for l-serine dehydratase in polyacrylamide gels

✍ Scribed by Frank Gannon; Kenneth M. Jones

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 167 KB
Volume: 79
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90438-9

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✦ Synopsis

An activity stain for the detection of L-serine dehydratase in gels is described. After electrophoresis, the gel is incubated with L-serine, and the pyruvate formed is detected by its reaction with 0.6% 1,2-diamino4nitrobenzene in 2 M WC1 at 90°C. A red band on a yellow background indicates the position of L-serine dehydratase.

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